Tillsonburg is located in the heart of Southwestern Ontario just south of Highway 401 and directly on Provincial Highway 3.

It is connected to the 401 by Highway 19 and enjoys quick and easy access to the remainder of Ontario and the United States through either of these routes.

Tillsonburg is located midway between the border crossings of Windsor/Detroit, Sarnia/Port Huron and Fort Erie/Buffalo. This provides a distinct advantage for companies locating here.

Highways 19, 59/13 and 3 provide access to Ontario's South Coast and the Long Point recreation area, which has been recognized as a United Nations' Biosphere Reserve.

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